American Society of Hematology

Fig. 7.

$Fig. 7. GM-CSF binds directly to βc. / Purified sβc was titrated (0 to 20 μM) against 5 μM GM-CSF (A) or 5 μM E21R (B). Reaction mixes were set up with 0 (dashed black), 1 μM (thin black), 2.5 μM (medium gray), 5 μM (medium black), 10 μM (thick gray), or 20 μM (thick black) sβc, incubated at 25°C for 2 hours, and fractionated by size-exclusion chromatography.$

GM-CSF binds directly to βc.

Purified sβc was titrated (0 to 20 μM) against 5 μM GM-CSF (A) or 5 μM E21R (B). Reaction mixes were set up with 0 (dashed black), 1 μM (thin black), 2.5 μM (medium gray), 5 μM (medium black), 10 μM (thick gray), or 20 μM (thick black) sβc, incubated at 25°C for 2 hours, and fractionated by size-exclusion chromatography.

This Feature Is Available To Subscribers Only