Fig. 2.
CBMCs cocultured with 3T3 fibroblasts increased their responsiveness to MBP.
CBMCs (> 90% mast cells) were maintained in suspension (■) or in coculture with 3T3 fibroblasts (▪) in the presence of soluble SCF (100 ng/mL) for 4 days before activation. (A) Histamine release was assessed after 30 minutes of incubation with the activators at the concentrations indicated. Data are the means ± SEM of 5 experiments performed in triplicate. (B-C) PGD2 production and release was assessed after 30 minutes of incubation with the activators at the concentrations indicated. Data are the means ± SEM of 2 experiments performed with CBMCs from 2 different donors.P < .05 compared with cells maintained in suspension.
