β-Galactosidase expression after prenatal intravenous delivery of adenoviral vector.

AdRSVLacZ was injected intravenously into prenatal mice 16 days after conception to examine distribution of β-galactosidase expression by this route. X-gal staining was performed on fetal sagittal sections 2 days after injection (A) or on whole neonatal liver 9 days after injection (B). Cellular expression in hepatocytes is confirmed on wax-embedded histologic sections of the stained whole liver 2 days after injection (original magnification, × 400) (C). Neutral red was used as counterstain.