Targeting of lymphohematopoietic cells by anti-CD45 antibody in vivo.

Twenty-four hours after a single injection of MAb, the peripheral blood (A) and bone marrow (B) were incubated with an FITC-labeled antirat antibody and analyzed by flow cytometry (shaded curve). Controls consisted of peripheral blood or bone marrow from untreated mice that was stained with rat secondary FITC antibody (solid line, unshaded) and of unstained cells (dotted line). For the detection of anti-CD45 antibody to the tissues in vivo, cryostat sections of a spleen 24 hours after antibody treatment (C) and of an untreated control (D) were stained with the rat FITC antibody, and the membrane-bound fluorescence was visualized by fluorescence microscopy (original magnification × 40; enlargements [original magnification × 160] inserted).