Fig. 3.
In vivo binding of anti-CD45 antibody 30F11 to hematopoietic progenitor cells.
Bone marrow of mice that had received a single dose (1 μg/g body weight) of anti-CD45 MAb 24 hours earlier was analyzed on a flow cytometer after double staining with a rat antibody and antibodies against the stem cell antigens Sca-1 (C) and CD34 (D). Negative and positive controls are isotype PE (A) and CD45.1 PE (B), respectively. Numbers in each quadrant represent percentages of total cells in each.