Fig. 7.
![Fig. 7. Cooperative transcriptional activation of the humanMBP promoter by C/EBPε32, GATA-1, and PU.1. / NIH 3T3 cells were cotransfected with combinations of expression vectors indicated on the x-axis. In addition, a human MBP-P2 promoter-luciferase construct [pMBP(-117)–LUC] was included in each transfection.30 At 24 hours after transfection, lysates were prepared, and luciferase activity (RLU) was measured and normalized to Renilla luciferase to control for transfection efficiency. The graph represents the average (± SD) of 2 experiments performed in triplicate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/8/10.1182_blood-2002-04-1039/4/h80834157007.jpeg?Expires=1735548936&Signature=YqRDEXGzD4M1XAxqY7dcE35sHp0vtEO6IYUo6X6G8RIDDH4Q45cvGXPm0xR610kB0mXslXULxWIUVj1T4fG2ZLsq8FXZSM2ZmcO-SA7vRLPvdZ1hww6eChRU1R8CFkJ2Nd7fbRTamOa25qJ4wNqMX8qUKKNeQ8j49h1fdFz4zkvzqwgTdjJTaj5VfB2pl6DGMtqeatC~M76QSftAAiKbzy-~DaIMiaYAY843pX3tzyKz6HnNEa~2mDeKJAie405yatbZePT3o7TXSuMCk3V3g0D7pcUwJbgynjVXyOs2CjiBegsZVPFrfKwv3alpiVmWJulg4zwM4UYVRGJ8i4pNdQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cooperative transcriptional activation of the humanMBP promoter by C/EBPε32, GATA-1, and PU.1.
NIH 3T3 cells were cotransfected with combinations of expression vectors indicated on the x-axis. In addition, a human MBP-P2 promoter-luciferase construct [pMBP(-117)–LUC] was included in each transfection.30 At 24 hours after transfection, lysates were prepared, and luciferase activity (RLU) was measured and normalized to Renilla luciferase to control for transfection efficiency. The graph represents the average (± SD) of 2 experiments performed in triplicate.
