Fig. 5.
Antagonism of G-CSF stimulation requires enzymatically active elastase.
CD34+ cells (105/mL) were cultured in the presence of 10 ng/mL G-CSF, with or without 1 μg/mL elastase. After 10 days of culture, nucleated cells were counted. (A) Abrogation of the blocking effect of elastase by PMSF or heat inactivation. Data are representative of 3 independent experiments. (B) Increased dose of G-CSF does not override inhibition by elastase (mean ± SEM of triplicates) (*P < .001).
