Digestion of G-CSF by elastase.

(A) Two micrograms cytokine incubated with or without 40 ng elastase for 15 minutes and analyzed by gel electrophoresis. Data are representative of 3 independent experiments. (B) Two micrograms G-CSF or fibronectin incubated with 40 ng elastase, cathepsin G, 500 ng proteinase 3, or without protease for 15 minutes and analyzed by gel electrophoresis. Lanes 4, 9, and 10 show 3 bands corresponding to proteinase 3 in addition to the degradation product of G-CSF (lane 4) and fibronectin (lane 9). Data are representative of 3 independent experiments.