B lymphocyte IgG secretion is increased in the presence of IVIGs.
Purified human B lymphocytes were cultured with IL-4 or IL-10 and irradiated L4.5 during 19 days with 4 mM glycine and 10 mg/mL BSA or with 10 mg/mL dialyzed IVIGs. Cells were extensively washed and transferred without IVIGs in complete culture medium and allowed to secrete for 6 hours in ELISPOT assay and 6 hours (IL-10) or 22 hours (IL-4) for ELISA evaluation of IgG and IgM contents. Secreted IgG (A) and IgM (B) amounts are expressed in nanograms per 1 × 106 cells per hour, and the proportion of cells secreting IgG (C) and IgM (D). Error bars show standard deviation for 2 dilutions in triplicate. Results are representative of 3 independent experiments. Both IVIG preparations showed similar results.
