B lymphocytes cultured in the presence of IVIGs secrete antibodies reacting with self-antigens.

All tested samples were adjusted to 100 ng/mL IgG. IgG reactivities for actin, insulin, myoglobin, KLH, histone, nucleoprotamine, and dsDNA were evaluated by ELISA as described in “Materials and methods.” IgG reactivities in commercial IVIGs (░), commercial human autoimmune anti-dsDNA serum (▧), and supernatants from B lymphocytes cultured with 10 mg/mL BSA and 4 mM glycine (■) or with 10 mg/mL dialyzed IVIGs (▪) are shown. Background reactivities (▤) for each antigen are also shown. Error bars show standard deviation for triplicates. Results are representative of 3 independent experiments and reactivity patterns are similar for both IVIG preparations.