Myc5 B-lymphoma cells acquire myeloid phenotype in vitro.

(A) Histolopathologic (left) and cytologic (right) analyses of Myc3 and Myc5 lymphomas maintained in vivo and in vitro, respectively. On the left, sections of tumor tissues were stained with H&E (original magnification, × 40). On the right, cultured cells were spun onto slides and stained with Wright-Giemsa (HemoQuick, original magnification, × 60). (B) Flow cytometric analyses of surface markers on primary (in vivo) and cultured (in vitro) Myc3 and Myc5 cells. Cells stained with specified antibodies are indicated by black histograms; control unstained cells, with open lines. + and − refer to positive and negative staining, respectively.