Fig. 2.
Fig. 2. Agonist-stimulated colocalization of internalized CXCR2 with EGFP-Rab11a in HEK293 cells and RBL-2H3 cells. / HEK293 cells (A-D) and RBL-2H3 cells (E-F) stably expressing CXCR2 were transiently transfected with plasmids encoding EGFP-Rab11a. Cells were treated with 200 nM CXCL8 at 37°C for different times as indicated, then fixed in methanol. Cells were incubated with a mouse monoclonal anti-CXCR2 antibody for 30 minutes, followed by incubation with a rhodamine-conjugated antimouse antibody for 30 minutes. Representative laser-scanning confocal micrographs from 3 independent experiments demonstrating the distribution of CXCR2 (red), EGFP-Rab11a (green), and colocalization of CXCR2 with EGFP-Rab11a (yellow) are shown. Images were processed using Photoshop software. Bars, 10 μm.

Agonist-stimulated colocalization of internalized CXCR2 with EGFP-Rab11a in HEK293 cells and RBL-2H3 cells.

HEK293 cells (A-D) and RBL-2H3 cells (E-F) stably expressing CXCR2 were transiently transfected with plasmids encoding EGFP-Rab11a. Cells were treated with 200 nM CXCL8 at 37°C for different times as indicated, then fixed in methanol. Cells were incubated with a mouse monoclonal anti-CXCR2 antibody for 30 minutes, followed by incubation with a rhodamine-conjugated antimouse antibody for 30 minutes. Representative laser-scanning confocal micrographs from 3 independent experiments demonstrating the distribution of CXCR2 (red), EGFP-Rab11a (green), and colocalization of CXCR2 with EGFP-Rab11a (yellow) are shown. Images were processed using Photoshop software. Bars, 10 μm.

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