CD95L-DCs induce strong T-cell proliferation in vivo.

(A) bm12 mice received one footpad injection of 10⁶ lpr/lpr DCs transduced with CD95L (black bars) or control retrovirus (gray bars), or were untreated (white bars). The total number of popliteal LN cells was determined 5 days later. Results were expressed as mean number of cells ± SEM (*P < .02). (B) bm12 mice were injected with 10⁶ lpr/lpr CD95L (black bar) or control DCs (gray bar). DNA was extracted from popliteal LNs 5 days later, and level of lpr mutation was measured by PCR. Results are expressed as mean of lpr versus β-actin signals ± SEM. Each group contains 4 individual mice. NS indicates not significant.