Fig. 8.
Intraperitoneal neutrophil recruitment by CD95L-DCs.
(A) Bm12 or lpr/lpr bm12 mice were injected intraperitoneally with either 8 × 105 control or CD95L-DCs. PECs were analyzed 16 hours later by flow cytometry. 200 μg of RB6-8C5 (anti-Gr1) mAbs were inoculated 4 hours before CD95L-DC treatment. (B) PEC cytospin preparation from CD95L-DC– (panel i) or control DC– (panel ii) injected mice were stained with May-Grünwald-Giemsa solution to identify polymorphonuclear leukocytes (arrow). Original magnification, × 400. Experiments were repeated at least 5 times with similar results.
