Sequencing strategy for total mtDNA.

Bars (–) show the 20 amplified mtDNA fragments. Numbers (1-40) indicate sequencing primers corresponding to the PCR products. The 40 sequence primers26 include 1 (F15), 2 (R921), 3 (F873), 4 (F1234), 5 (F1657), 6 (F2105), 7 (F2972), 8 (R3006), 9 (F3441), 10 (F3931), 11 (F4447), 12 (F4976), 13 (F5318), 14 (F5700), 15 (R6526), 16 (F6426), 17 (R7255), 18 (F7215), 19 (F7645), 20 (F8164), 21 (F8539), 22 (F9309), 23 (R9403), 24 (F9754), 25 (F10127), 26 (F11001), 27 (R11166), 28 (R11927), 29 (F11901), 30 (F12357), 31 (F12793), 32 (F13188), 33 (F13518), 34 (R14388), 35 (R14926), 36 (R15396), 37 (F15260), 38 (R16451), 39 (F16097), and 40 (R336). Original primer names are shown in parentheses.