Comparison of the effect of G6PT inhibitors in microsomes from human PMNs, differentiated HL-60 cells, and rat liver.

Microsomes from the indicated cell types were preincubated with 10 μM S3483 for 5 minutes at 22°C, 0.5 mM DIDS for 10 minutes at 22°C, or treated with 1 mM N-ethylmaleimide (NEM) as described in Fulceri et al.33 G6P uptake (and the case of liver microsomes, also intravesicular glucose accumulation) was then assayed radioisotopically by rapid filtration. Data are expressed as percent of inhibition. Values are means ± SEMs of 3 to 5 independent experiments.