S3483 compound inhibits cell growth and induces apoptosis in HL-60 cells undergoing differentiation.

In the left panel, HL-60 cells were cultured in RPMI 1640 complete medium containing 1.25% (vol/vol) DMSO to promote cell differentiation, and including also 100 μM S3483 ▵ or solvent alone (10 μL/mL ethanol, ■); the apoptotic ratio of S3483-treated versus untreated cells, calculated by measuring the levels of cytoplasmic histone-associated DNA fragments with a Cell Death Detection ELISA^plus (Roche) kit, is also shown (●). In the middle and right panels, HL-60 and Jurkat cells were cultured in RPMI 1640 complete medium containing 100 μM S3483 ▵ or solvent alone (10 μL/mL ethanol, ■). Values are means ±SEM of 3 to 5 independent experiments.