Induction of IL-8 expression in FPRL1/LXA4R-transfected cells.

(A) HeLa cells were transiently transfected with vector (mock) or a FPRL1/LXA4R expression construct, together with one of the luciferase reporters as indicated. Two days after transfection, cells were stimulated with SAA and the expressed luciferase activities were determined. Data shown are mean ± SEM from 3 separate experiments, each performed in duplicate. (B) The cell surface expression of the receptor was confirmed in FPRL-transfected HeLa cells (open curve), but not in vector (pRK5)–transfected HeLa cells (shaded curve), by flow cytometry using anti-FPRL1 and FITC-conjugated secondary antibody.