Development of myelofibrosis in the spleen of TPO-overexpressing mice.

Representative spleen sections from WT andopg^−/− hosts hematologically repopulated with TPO virus–infected BM cells from WT or opg^−/−donors at 3 months after transplantation. Sections were stained according to Gordon-Sweet to reveal reticulin fiber deposition. (A) WT host engrafted with WT BM cells. (B) opg^−/−host engrafted with opg^−/− BM cells. (C) WT host engrafted with opg^−/− BM cells. (D)opg^−/− host engrafted with WT BM cells. Sections show severe accumulation of reticulin fibers in the 4 experimental groups. Original magnification for all panels, × 200.