Immobilized Delta-1–FL inhibits osteoclast development.

Freshly prepared BM cells, spleen cells, and cells from the peritoneal cavity (PECs) were cultured on dishes coated with Delta-1–FL or BAP-FL in the presence of RANKL (25 ng/mL) and M-CSF (50 ng/mL). After 6 days of culturing, the number of TRAP⁺-MNCs was counted. Bars indicate means ± SDs of triplicate cultures. *P < .05 and **P < .005 indicate significantly different from the corresponding cultures in dishes coated with BAP-FL.