American Society of Hematology

Fig. 7.

$Fig. 7. FACS analysis of splenic erythroid populations in long-term chimeras. / Y-axis: Ter-119 expression (BD PharMingen); X-axis: cell size (forward scatter). The left box corresponds to the smaller, more mature fraction of erythroid cells. Ter119+ cells represented, respectively, 47% ± 10% and 79% ± 18% of all spleen cells in eGFP-Hbb+/+ (n = 5) and eGFP-Hbbth3/+ (n = 5) chimeras. In recipients of eGFP-transduced Hbbth3/th3(n = 4), erythroid cells represented 93% ± 2% of splenocytes, and in TNS9-transduced Hbbth3/th3, 93% ± 5% (n = 4).$

FACS analysis of splenic erythroid populations in long-term chimeras.

Y-axis: Ter-119 expression (BD PharMingen); X-axis: cell size (forward scatter). The left box corresponds to the smaller, more mature fraction of erythroid cells. Ter119⁺ cells represented, respectively, 47% ± 10% and 79% ± 18% of all spleen cells in eGFP-Hbb^+/+ (n = 5) and eGFP-Hbb^th3^/+ (n = 5) chimeras. In recipients of eGFP-transduced Hbb^th3/th3(n = 4), erythroid cells represented 93% ± 2% of splenocytes, and in TNS9-transduced Hbb^th3/th3, 93% ± 5% (n = 4).

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