Reactivation of SOCS-1 expression by DAC treatment in MM cell lines.

Cell lines XG1 and U266 were incubated with or without 1.0 μM DAC in complete medium for 96 hours. Peripheral blood mononuclear cells (PBMNCs) after stimulation with IL-6 for 2 hours served as positive control. The presence or absence of reverse transcriptase is indicated by RT⁺ or RT⁻, respectively. RT-PCR for GAPDH was performed for all samples to ensure the integrity of the reverse transcription reactions.