Fig. 5.
Immunofluorescence images of ECs cultured on OP9 without exogenous factors.
(A-I) In the absence of exogenous factors, sheetlike clusters were formed. ECs were polygonal and VE-cadherin, γ-catenins, and PECAM-1 were colocalized at stringlike cell-cell junctions (ZO1–VE-cadherin [VECD-1], panels A-B; γ-catenins–VE-cadherin [VECD-1], panels D-E; PECAM-1–VE-cadherin [VECD-1], panels G-H). Distributions of adhesion molecules were compared in merged images (panels C, F, I). Scale bar, 20 μm.