Fig. 7.
![Fig. 7. Intracellular localization of VE-cadherin in VECD-1–treated ECs. / (A-F) The addition of 20 μg/mL VECD-1 disrupted EC-EC contact, thereby allowing ECs to scatter. VE-cadherin was translocated from cell surfaces to the intracellular cytoplasm (panel E, arrow). Internalized VE-cadherin colocalized with γ-catenin (panel A, arrow) (γ-catenin–VE-cadherin [VECD-1]; panels A-B). (D-F) In contrast, ZO1 remains at the cell-cell junctions (ZO1–secondary Ab; panels D-E). Panels C and F are merged images. Scale bar, 20 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/4/10.1182_blood-2002-05-1329/3/h80433817007.jpeg?Expires=1735840593&Signature=VTkk4VPF32KXEL~JXyr-UIXhMZyKuKLNbMfSmLzJDzctDZka54RFcvac3cXCDLqLG-BXZ0MtcLDE52~N2CtLKQx63~5MpLWpW0AVCEXc~idBCe3qkQgaWD7jR3CCSFecoQlxwrEyHSFERPJBgPgVNhNFuIU8aKGMlotCCvYSppfvfEEf-OG09FUBAStFa3JSSjMwb2BYHJcT~7l1QkshkP8jaEYNcdUAPqSFik6a-uo9leugApPkle8Hsgwaibijlc5x2ATHSZoNnQJtLgoC3bSJLby4t-ltSO7UmN5lY9kkPaopdsfraL~MdXGuSnFO51tbrddhgi74Zt6HidBavQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Intracellular localization of VE-cadherin in VECD-1–treated ECs.
(A-F) The addition of 20 μg/mL VECD-1 disrupted EC-EC contact, thereby allowing ECs to scatter. VE-cadherin was translocated from cell surfaces to the intracellular cytoplasm (panel E, arrow). Internalized VE-cadherin colocalized with γ-catenin (panel A, arrow) (γ-catenin–VE-cadherin [VECD-1]; panels A-B). (D-F) In contrast, ZO1 remains at the cell-cell junctions (ZO1–secondary Ab; panels D-E). Panels C and F are merged images. Scale bar, 20 μm.
