Fig. 3.
KDR and Flt1 mRNA and protein expression.
Representative results of mRNA expression on freshly purified HPCs (A); HPC-generated precursors differentiating and maturing through the Mk and E (B) and Mo and G (C) pathways in unilineage cultures. Aliquots of reverse transcribed RNA from 2 to 5 × 104 cells were collected at the indicated days and amplified by 40 PCR cycles based on β2-microglobulin normalization. (D) Western blot analysis of 50 μg/lane protein lysates from day 5 and day 7 megakaryocytes; K562 and HeLa cell lines were used as positive and negative control, respectively.
