The multilineage reconstitution abilities of CD34⁺ SRCs and IBMI-CD34⁻ SRCs.

First, the R1 gate was set on the total murine BM cells obtained from these representative mice 12 weeks after the transplantation of 5 × 10³ Lin⁻CD34^high or 5 × 10⁴ Lin⁻CD34⁻ cells, and then human CD45⁺ cells were gated as R2. Expression of lineage markers, including CD19 (pan-B cell), CD33 (myeloid), CD14 (monocytic), and CD41 (megakaryocytic) on the R2-gated cells was analyzed by 3-color flow cytometry. Only the expression of GPA (erythroid) was analyzed on the whole BM cells (no gate). In this particular mouse that received a transplant of CD34⁺ SRCs (left column), 39.2% of total BM cells were human CD45⁺cells, which contained 11.0% of CD34⁺ cells, 50.1% of CD19⁺ cells, 5.7% of CD33⁺ cells, 5.3% of CD14⁺ cells, and 2.4% of CD41⁺ cells. In addition, 1.4% of whole murine BM cells were human GPA⁺cells. In another representative mouse that received a transplant of IBMI-CD34⁻ SRCs (right column), 52.4% of total BM cells were human CD45⁺ cells, which contained 13.0% of CD34⁺ cells, 19.1% of CD19⁺ cells, 4.0% of CD33⁺ cells, 3.2% of CD14⁺ cells, and 1.9% of CD41⁺ cells. In addition, 0.5% of whole murine BM cells were human GPA⁺ cells.