Comparison of the differentiation potential of IBMI-CD34⁻ SRCs with that of CD34⁺SRCs.

The multilineage reconstitution abilities of IBMI-CD34⁻(gray columns) and CD34⁺ (open columns) SRCs using IBMI technique were compared. Each bar represents the median of positive rates obtained from 3 mice that received transplants of either 5 × 10³ Lin⁻CD34^high or 5 × 10⁴ Lin⁻CD34⁻ cells. In human CD45⁺ cells, the rate of CD34⁺ cells was almost comparable in both populations. On the other hand, the rates of CD19⁺, CD33⁺, CD14⁺, and CD41⁺ in human CD45⁺ cells and GPA⁺cells in whole BM cells were significantly (P < .05) higher in the mice that received transplants of CD34⁺ SRCs than in those that received IBMI-CD34⁻ SRCs.