Immunoblots of platelet αIIb and β3 for patients M and C.

Platelets were solubilized in SDS, electrophoresed into a 7% polyacrylamide gel under reduced (αIIb) and nonreduced (β3) conditions, and electrotransferred to PVDF membranes. Equal amounts of protein were electrophoresed as judged by the staining of the gel after electrophoresis for platelet myosin heavy chain (Mr about 200 000). Membranes analyzed for αIIb were incubated with the mAb PMI-1, and membranes analyzed for β3 were incubated with the mAb 7H2.28 (A) Samples from patient M and his mother and father. (B) Samples from patient C and his 2 siblings.