Fig. 2.
Fig. 2. Bcl-XL antisense induces premature apoptosis in DMSO-induced MEL cells. / (A) Cell viability of MEL cells in the absence or in the presence of DMSO. 745A/Neo and 745A/Bcl-XLAS cells were seeded at 105 cells/mL with or without 2% DMSO. At the times indicated (days) after growth initiation, cell viability was measured by trypan blue dye exclusion. Data represent the mean of triplicate cultures ± standard deviation. (B) DNA fragmentation analyzed by gel electrophoresis followed by staining with ethidium bromide in MEL cells treated with DMSO for indicated times (days). Lane 1: 745A/Neo cells; lane 2: 745A/Bcl-XLAS cells. (C) DNA breaks determined by TUNEL assay in MEL cells treated with DMSO for indicated days. Numbers on the horizontal bar indicate apoptotic cells as the percent of total cell number.

Bcl-XL antisense induces premature apoptosis in DMSO-induced MEL cells.

(A) Cell viability of MEL cells in the absence or in the presence of DMSO. 745A/Neo and 745A/Bcl-XLAS cells were seeded at 105 cells/mL with or without 2% DMSO. At the times indicated (days) after growth initiation, cell viability was measured by trypan blue dye exclusion. Data represent the mean of triplicate cultures ± standard deviation. (B) DNA fragmentation analyzed by gel electrophoresis followed by staining with ethidium bromide in MEL cells treated with DMSO for indicated times (days). Lane 1: 745A/Neo cells; lane 2: 745A/Bcl-XLAS cells. (C) DNA breaks determined by TUNEL assay in MEL cells treated with DMSO for indicated days. Numbers on the horizontal bar indicate apoptotic cells as the percent of total cell number.

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