Fig. 5.
Fig. 5. Bcl-XL antisense oligonucleotide does not induce apoptosis but inhibits hemoglobin synthesis in MEL cells overexpressing Bcl-2. / 745A/Neo and 745A/Bcl-2 cells were seeded at 105 cells/mL and cultured in the presence of 2% DMSO with or without Bcl-XL antisense oligonucleotide (Bcl-XL-AS). At 5 days after DMSO treatment, the following parameters were determined: (A) percentage of apoptotic cells measured by staining cells with a mixture of ethidium bromide (EB) and acridine orange (AO) (data represent the mean of quadruplicate cultures ± standard deviation); (B) proportion of differentiated cells measured by benzidine staining (data represent the mean of quadruplicate cultures ± standard deviation).

Bcl-XL antisense oligonucleotide does not induce apoptosis but inhibits hemoglobin synthesis in MEL cells overexpressing Bcl-2.

745A/Neo and 745A/Bcl-2 cells were seeded at 105 cells/mL and cultured in the presence of 2% DMSO with or without Bcl-XL antisense oligonucleotide (Bcl-XL-AS). At 5 days after DMSO treatment, the following parameters were determined: (A) percentage of apoptotic cells measured by staining cells with a mixture of ethidium bromide (EB) and acridine orange (AO) (data represent the mean of quadruplicate cultures ± standard deviation); (B) proportion of differentiated cells measured by benzidine staining (data represent the mean of quadruplicate cultures ± standard deviation).

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