Interaction of ABC7 with ferrochelatase.

(A) Mitochondrial localization. Cos7 cells were cotransfected with pcDNA3(c-myc)-ABC7 and pCD-MF and incubated for 44 hours. They were then fixed, permeabilized, and reacted simultaneously with anti–c-Myc and antiferrochelatase to show colocalization of ABC7 (green) and ferrochelatase (red). The merged exposure (yellow) confirms that the dots colocalize. (B) Immunoblots of extracts from Cos7 cells transfected with pcDNA3(c-myc)-ABC7 and pCD-MF. The cells transfected with pCD-MF (lanes 1 and 3) or pcDNA3(c-myc)-ABC7 (lanes 2 and 3) were cultured for 24 hours. Immunoblots were performed with anti–c-Myc (top panel) and antiferrochelatase (bottom panel). (C) Extracts from panel B were subjected to immunoprecipitation with anti–c-Myc, followed by immunoblotting with antiferrochelatase. (D) Extracts from panel B were subjected to immunoprecipitation with antiferrochelatase, followed by immunoblotting with anti–c-Myc.