Fig. 4.
Fig. 4. Activities and levels of ferrochelatase, catalase, and iron-sulfur–containing proteis in ABC7 cDNA-transfected BNL-CL2 cells. / (A) Ferrochelatase activity. Mouse embryo liver BNL-CL2 cells were transfected with empty vector (■) or pcDNA(c-Myc)-ABC7 plasmids (▪) and incubated for 24 and 48 hours. The cells were then collected, washed twice with PBS, and homogenized. The homogenates were centrifuged at 900g for 10 minutes. The assay of ferrochelatase activity was as described. (B) Catalase activity. Catalase activity was measured using homogenates as above. Data in panels 4A-B are expressed as the means ± SDs of triplicate experiments. (C) Immunoblot analysis. Cells transfected with empty vector or pcDNA3-ABC7 were cultured. The cellular proteins were analyzed by SDS-PAGE, transferred onto PVDF membrane, and immunoblotted using antiferrochelatase, anticatalase, and antithioredoxin.

Activities and levels of ferrochelatase, catalase, and iron-sulfur–containing proteis in ABC7 cDNA-transfected BNL-CL2 cells.

(A) Ferrochelatase activity. Mouse embryo liver BNL-CL2 cells were transfected with empty vector (■) or pcDNA(c-Myc)-ABC7 plasmids (▪) and incubated for 24 and 48 hours. The cells were then collected, washed twice with PBS, and homogenized. The homogenates were centrifuged at 900g for 10 minutes. The assay of ferrochelatase activity was as described. (B) Catalase activity. Catalase activity was measured using homogenates as above. Data in panels 4A-B are expressed as the means ± SDs of triplicate experiments. (C) Immunoblot analysis. Cells transfected with empty vector or pcDNA3-ABC7 were cultured. The cellular proteins were analyzed by SDS-PAGE, transferred onto PVDF membrane, and immunoblotted using antiferrochelatase, anticatalase, and antithioredoxin.

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