The amount of heme and levels of ferrochelatase and thioredoxin in Me₂SO-induced ABC7-expressing MEL cells.

Cells expressing ABC7 and empty vector (5 × 10⁵/mL each) were incubated with 2% Me₂SO. (A) At the times indicated, the cells expressing ABC7 (○) and control (●) cells were collected and the amount of heme was measured. Data are expressed as the means ± SDs of triplicate experiments. (B) Immunoblot analysis. Immunoblotting was performed using antiferrochelatase and antithioredoxin. The transfected and control cells were separately cultured with 2% Me₂SO for 0 hours and 72 hours.