The generation of DCs from BM cells is impaired in TNF/LTα/LTβ^−/− mice.

BM cells (1 × 10⁶ cells/mL) taken from either wt or TNF/LTα/LTβ^−/− mice were cultured with GM-CSF and IL-4 for 9 days as described in “Materials and methods.” Cells were collected on day 9 and stained with FITC-conjugated anti–I-A^b mAb and PE-conjugated anti-CD11c mAb. The percent of events in each quadrant of dot plots is indicated. Dots were plotted based on staining with isotype-matched negative control mAb. Data from 1 of 5 representative experiments are shown.