Fig. 3.
![Fig. 3. The stimulatory activity of cells generated from TNF/LTα/LTβ−/− BM culture is significantly reduced compared with that from wt BM culture. / BM cells (1 × 106 cells/mL) from either wt (●) or TNF/LTα/LTβ−/− (○) mice were cultured with GM-CSF and IL-4 for 9 days. Cells were collected on day 9 and subjected to 30 Gy irradiation. Indicated number of generated cells were cocultured with syngeneic (A) or allogeneic (B) LN cells (1 × 106 cells/mL) for 4 days. During the last 18 hours of the culture, [3H]dThd was added in each well. The values are the means ± SD of quadruplicate wells. Data from 1 of 3 representative experiments are shown. *P < .01 compared with TNF/LTα/LTβ−/− BM culture. CPM indicates counts per minute.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/4/10.1182_blood.v101.4.1477/3/h80433854003.jpeg?Expires=1735554734&Signature=KgGdvbA2COB~ZLiVSGTwYUVH-GC1Hhbgpe1c7jqjZ8Dd8~mLWxnZueNr3OuZx8MKQe2PMVKk5RE7RHzMO6mbnlRylmgp4a5iOqroIFAeygkbeim~rSB2NJYlSrITdUEH53a~4G27l8NFMljZfbtqQyNtwjYDVnP~jpPQucJNPaa0Ie6RjyTvnxCGwqaeTi-K1iulTPbpzz5Ui1xkS-IaNpw0TsnWzOChEN092MCzVPHtmlKuuwiwO1ROQHY2FWg12knPPX9D6EynSJQ15INkN0dzIYISVC5UqxR~o1ldOukqS0XESCpWZs7Ty2b76OqFGOnJMmse4gZ63XdkyB-tEw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The stimulatory activity of cells generated from TNF/LTα/LTβ−/− BM culture is significantly reduced compared with that from wt BM culture.
BM cells (1 × 106 cells/mL) from either wt (●) or TNF/LTα/LTβ−/− (○) mice were cultured with GM-CSF and IL-4 for 9 days. Cells were collected on day 9 and subjected to 30 Gy irradiation. Indicated number of generated cells were cocultured with syngeneic (A) or allogeneic (B) LN cells (1 × 106 cells/mL) for 4 days. During the last 18 hours of the culture, [3H]dThd was added in each well. The values are the means ± SD of quadruplicate wells. Data from 1 of 3 representative experiments are shown. *P < .01 compared with TNF/LTα/LTβ−/− BM culture. CPM indicates counts per minute.
