B-ALL and lymphoma CD19⁺ tumor lines differ in relative expression of T-cell adhesion molecules.

Cell surface expression of molecules that participate in activation of CD19-specific T cells assessed by flow cytometry on a panel of CD19⁺B-ALL and lymphoma lines (A) and a genetically modified T cell (B). Relative expression of the adhesion molecules and CD19 (solid lines) within the gate M2 is reported as a percentage and was determined based on binding of the specific mAbs compared with isotype-matched controls (dotted lines). The MFI for the cells in gate M2 reflects the relative density of adhesion molecules expressed on the cell surface. Dead cells were excluded on uptake of PI.