Effect of in vivo CTLA4Ig treatment on MLRs of splenocytes and T cells.

Splenocytes were harvested on day 10 after transplantation from CTLA4Ig-treated (♦) or control (▵) LEW.1A rats that received LEW.1W heart transplants. Total splenocytes or T cells isolated from splenocytes of the same animal were assayed against donor (LEW.1W), third-party (BN), or F1 LEW.1W × LEW.1A APCs at the indicated time points. (A) Splenocyte proliferation against LEW.1W cells. (B) T-cell proliferation against BN cells. (C) Splenocyte proliferation against LEW.1W cells. (D) T-cell proliferation against BN cells. (E) Splenocyte proliferation against LEW.1W × LEW.1A cells. (F) T-cell proliferation against LEW.1W × LEW.1A cells. Results are expressed as delta (allogeneic − spontaneous) cpm ± SD. Results are representative of 3 experiments with identical results.