![Fig. 2. Effect of transwell cultures on inhibition of MLRs. / Splenocytes from naive LEW.1A animals were cultured in the upper compartment of transwell plates with irradiated LEW.1W APCs, and proliferation was evaluated on days 3 and 5 of culture. The lower compartment contained splenocytes from 2 CTLA4Ig-treated or 1 control LEW.1A rat that received LEW.1W heart transplants harvested on day 10 after transplantation and cultured with (A) culture medium, (B) irradiated LEW.1W APCs, or (C) irradiated BN APCs. (D) Splenocytes from naive LEW.1A animals were cultured in the upper compartment of transwell plates with BN irradiated APCs in the presence, in the lower compartment, of splenocytes from CTLA4Ig-treated or control LEW.1A rats and irradiated LEW.1W APCs. [3H]Tdr incorporation was assessed in the upper compartment MLR on days 3 and 5 of culture. Results are expressed as delta (allogeneic − spontaneous) cpm ± SD and are representative of 2 identical experiments with a total of 4 animals in each group. Each curve represents the proliferation of cells from a single animal.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/8/10.1182_blood-2002-07-2076/4/h80834180002.jpeg?Expires=1735825787&Signature=DQ~qtx4jVKaXN3OkKDMksk7ORzoUdlmKjLzC7ewqc59NMAM8khZgt7zAv2qNJoclx6B0swqa94fxsOBTBaN54uSCQHE7HwRFn5F2czfWtcGdNFh2gxg0PXsxUpVgIiZ~UGrCWut3PD0dGqaFcov4z2WWvgLPSx~7CTjpqLuVvWocwI~GVKEv54R4SNu8HB8C1wf0sveQv-Pv0z0Ui-4jwjesTdAVap-gcz87OYwxC45yA8KHGb2lAVKN9ulFUfRqGh-fFdQyckL8jWjz9hXnI5eYQk8QN2-8USnleM3CN3NLk1jrTcOemQl6oesafG6QsV2qALTkpIPk~gs8-~Iqrg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of transwell cultures on inhibition of MLRs.
Splenocytes from naive LEW.1A animals were cultured in the upper compartment of transwell plates with irradiated LEW.1W APCs, and proliferation was evaluated on days 3 and 5 of culture. The lower compartment contained splenocytes from 2 CTLA4Ig-treated or 1 control LEW.1A rat that received LEW.1W heart transplants harvested on day 10 after transplantation and cultured with (A) culture medium, (B) irradiated LEW.1W APCs, or (C) irradiated BN APCs. (D) Splenocytes from naive LEW.1A animals were cultured in the upper compartment of transwell plates with BN irradiated APCs in the presence, in the lower compartment, of splenocytes from CTLA4Ig-treated or control LEW.1A rats and irradiated LEW.1W APCs. [3H]Tdr incorporation was assessed in the upper compartment MLR on days 3 and 5 of culture. Results are expressed as delta (allogeneic − spontaneous) cpm ± SD and are representative of 2 identical experiments with a total of 4 animals in each group. Each curve represents the proliferation of cells from a single animal.
