Fig. 9.
Modulation of MAP kinase activity following exposure to EB1089.
A total of 1 × 10−8 M EB1089 induced marked phosphorylation of p38 MAP kinase and dephosphorylation of ERK after 8 hours in culture. The p38 inhibitor, SB203580, at a final concentration of 1μ M prevented EB1089-induced phosphorylation and downstream dephosphorylation of ERK. In contrast, the ERK inhibitor, PD98059, failed to prevent p38 MAP kinase phosphorylation despite effective inhibition of ERK. Data shown represent the mean (± SD) of duplicate experiments carried out on samples from 12 individual patients.
