Inhibition of AG490-induced apoptosis by the caspase inhibitor Z-VAD-fmk.

Cells were incubated with 100 μM Z-VAD-fmk for 1 hour and were then treated for 48 hours with 100 μM AG490 or 0.1% DMSO. Cells were stained with annexin V–FITC and PI, and analyzed for apoptosis by flow cytometry. Shown is a representative of 2 experiments performed.