Functional evaluation of the wt-A1 in flow-dependent platelet tethering.

(A) Glass coverslips coated with purified wt-A1 (300 μg/mL) were untreated (control) or treated for 30 minutes at 37°C with 1mM CuSO₄ or 10 mM DTE as indicated and were perfused with platelets in reconstituted blood at a wall shear rate of 1500 seconds⁻¹. After 3 minutes, interacting platelets were counted. Results are expressed as percentages of interacting platelets relative to the nontreated wt-A1 (*P < .01). (B) Images (205 × 330 μm) show the flow-dependent tethering of fluorescently labeled platelets on glass coverslips coated with wt-A1 (300 μg/mL) or native VWF (50 μg/mL) at 200 seconds⁻¹(i,vi), 1000 seconds⁻¹ (ii,vii), and 1500 seconds⁻¹ (iii,viii) and the inhibitory effect of 30 μg/mL G19H10 (iv,ix) and 20 μg/mL AJvW-2 (v,x) at 1500 seconds⁻¹. Corresponding platelet counts ± SDs are provided in subpanels.