American Society of Hematology

Fig. 5.

$Fig. 5. Heparin binding. / (A) Binding of wt-A1 and mutants to heparin. Fusion proteins (50 μg/mL) were incubated with heparin-Sepharose beads for 2 hours at 37°C. The bound fraction of the proteins was calculated from the concentration of unbound material measured after centrifugation of the beads. Results are expressed as mean percentages ± SDs of protein bound to the beads for triplicate determinations (*P < .01). A GST/human VWF A3 domain fusion protein was tested as a control for nonspecific binding to heparin-Sepharose beads. For space considerations, single-letter codes were used for amino acids. (B) Inhibition of wt-A1 binding to immobilized AJvW-2 by soluble unfractionated heparin during perfusions in a BIACore 1000 instrument.$

Heparin binding.

(A) Binding of wt-A1 and mutants to heparin. Fusion proteins (50 μg/mL) were incubated with heparin-Sepharose beads for 2 hours at 37°C. The bound fraction of the proteins was calculated from the concentration of unbound material measured after centrifugation of the beads. Results are expressed as mean percentages ± SDs of protein bound to the beads for triplicate determinations (*P < .01). A GST/human VWF A3 domain fusion protein was tested as a control for nonspecific binding to heparin-Sepharose beads. For space considerations, single-letter codes were used for amino acids. (B) Inhibition of wt-A1 binding to immobilized AJvW-2 by soluble unfractionated heparin during perfusions in a BIACore 1000 instrument.

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