Inhibition of mediator-induced GPIb-VWF interaction by wt-A1 and mutants.

(A) Botrocetin assay. Reciprocal IC₅₀ values for the competitive binding of the indicated A1 mutants, in comparison with wt-A1, during the binding of 0.5 μg/mL VWF to microtiter plate-coated glycocalicin, mediated by 2 μg/mL botrocetin. Results are expressed as relative inhibition (1/IC₅₀). (B) Inhibition of RIPA by wt-A1 and mutants conducted in PRP. Means ± SDs of triplicate determinations (*P < .05; **P < .01). For space considerations, single-letter codes were used for amino acids.