Fig. 4.
Fig. 4. BCL3 gene copies in ALCL cell lines. / (A) Southern blot analysis of DNA extracted from ALCL and HD cell lines. The membranes were sequentially hybridized with BCL3cDNA and BCL6 genomic DNA probes.HindIII-digested λ phage DNA was a molecular weight marker. (B) Chromosomal FISH analysis of Karpas 299 (i) and SU-DHL-1 (ii). Metaphase preparations were hybridized with a BAC clone containing the BCL3 locus, which was labeled with digoxigenin/antidigoxigenin-FITC. The chromosomes and nuclei were counterstained with propidium iodide (PI). The arrowheads indicate duplicated (i) and amplified (ii) BCL3 gene copies. (C) Interphase FISH analysis of Karpas 299 (i), SU-DHL-1 (ii), DEL (iii), and SR-786 (iv). Original magnification Bi-ii, × 1000; Ci-iv, × 1000.

BCL3 gene copies in ALCL cell lines.

(A) Southern blot analysis of DNA extracted from ALCL and HD cell lines. The membranes were sequentially hybridized with BCL3cDNA and BCL6 genomic DNA probes.HindIII-digested λ phage DNA was a molecular weight marker. (B) Chromosomal FISH analysis of Karpas 299 (i) and SU-DHL-1 (ii). Metaphase preparations were hybridized with a BAC clone containing the BCL3 locus, which was labeled with digoxigenin/antidigoxigenin-FITC. The chromosomes and nuclei were counterstained with propidium iodide (PI). The arrowheads indicate duplicated (i) and amplified (ii) BCL3 gene copies. (C) Interphase FISH analysis of Karpas 299 (i), SU-DHL-1 (ii), DEL (iii), and SR-786 (iv). Original magnification Bi-ii, × 1000; Ci-iv, × 1000.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal