HDAC-i influence on viability, cell cycle, and apoptosis.

PBL cultures were treated with or without the indicated amount of FR901228 and stimulated for 24 hours with 3 × 28 beads. Viability was measured as exclusion of PI by flow cytometry (A). Cell cycle distribution and apoptosis was measured by intracellular PI staining (B) as described in “Materials and methods.”