Calcium measurement after FR901228 pretreatment of CD3-activated PBL cultures.

Increase in intracellular calcium content was measured after anti-CD3 stimulation of Fura-2–loaded cells preincubated with or without 20 ng/mL FR901228. Thick line represents anti-CD3 stimulation; thin line, control stimulation with PBS. Stimulation was initiated 2 minutes after start of recording and is indicated by arrow.