Fig. 6.
Fig. 6. HDAC-i influence on IL-2 production from activated CD4 T cells. / PBL cultures were preincubated with or without 20 ng/mL FR901228 or 100 ng/mL trichostatin A (TSA) and stimulated for 24 hours with 3 × 28 beads. (A) IL-2 was measured in the supernatant by ELISA. (B) Intracellular IL-2 was measured by flow cytometry on gated CD4 cells.

HDAC-i influence on IL-2 production from activated CD4 T cells.

PBL cultures were preincubated with or without 20 ng/mL FR901228 or 100 ng/mL trichostatin A (TSA) and stimulated for 24 hours with 3 × 28 beads. (A) IL-2 was measured in the supernatant by ELISA. (B) Intracellular IL-2 was measured by flow cytometry on gated CD4 cells.

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