Fig. 6.
Fig. 6. Zimm plot for purified human VWF. / Unsheared VWF samples at 3 different concentrations (CA = 5.6, 22.3, and 44.5 μg/mL) were analyzed using SLS. Detector angles (θ) were varied from 35° to 145° with respect to the incident beam. Discrete points depict experimental data. Solid lines are used to fit the data at either constant VWF concentration (CA) or constant detector angles (θ). Data extrapolated toCA = 0 and θ = 0 are depicted by broken lines. The Zimm plot shown here can be used to determine the MWt, Rg, and A2. Hereα is an arbitrary constant that equals 69 000. MWt was estimated to equal 5.7 × 106 Da,Rg was 63.6 nm, and A2was −1.53 × 10−4 mL × mol/g2.

Zimm plot for purified human VWF.

Unsheared VWF samples at 3 different concentrations (CA = 5.6, 22.3, and 44.5 μg/mL) were analyzed using SLS. Detector angles (θ) were varied from 35° to 145° with respect to the incident beam. Discrete points depict experimental data. Solid lines are used to fit the data at either constant VWF concentration (CA) or constant detector angles (θ). Data extrapolated toCA = 0 and θ = 0 are depicted by broken lines. The Zimm plot shown here can be used to determine the MWt, Rg, and A2. Hereα is an arbitrary constant that equals 69 000. MWt was estimated to equal 5.7 × 106 Da,Rg was 63.6 nm, and A2was −1.53 × 10−4 mL × mol/g2.

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