RT-PCR analysis of thrombopoietin and c-mpl mRNA expression.

Whole embryos or embryo proper (ep) and yolk sac (ys) were dissected from timed-pregnant female mice. Total RNA was isolated and reverse transcribed into cDNA followed by PCR amplification by using specific primers for Tpo (220 bp) and c-mpl (368 bp). Products were run on 2% agarose gel, and the size was determined by a 100-bp DNA standard ladder. Data presented represent 1 of 3 experiments.