Tpo treatment modulates formation of EryP, BFU-E, CFU-MIX, and CFU-Meg in a dose-dependent fashion.

(A) E8.5 yolk sac cells were plated at 10 000 cells/mL in primitive erythroid colony assay containing various concentration of Tpo. (B) E9.5 yolk sac cells were plated 10 000 cells/mL in the progenitor assay or megakaryocyte assay containing various concentrations of Tpo. *P < .05, various concentrations of Tpo compared with no Tpo. Data represent the mean values of triplicate samples from 4 experiments; error bars represent SEM.